5KIU
VCP-interacting membrane protein (VIMP)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-01 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 121.729, 17.973, 33.993 |
| Unit cell angles | 90.00, 95.58, 90.00 |
Refinement procedure
| Resolution | 60.580 - 2.200 |
| R-factor | 0.1885 |
| Rwork | 0.186 |
| R-free | 0.24434 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2q2f |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.943 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.580 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.286 | |
| Number of reflections | 3854 | |
| <I/σ(I)> | 11 | |
| Completeness [%] | 92.0 | |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 288 | 0.1 M MES, pH 6.0, 20 % 2-propanol, 20 % PEG MME 2000 |
