5KIU
VCP-interacting membrane protein (VIMP)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-08-01 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 121.729, 17.973, 33.993 |
Unit cell angles | 90.00, 95.58, 90.00 |
Refinement procedure
Resolution | 60.580 - 2.200 |
R-factor | 0.1885 |
Rwork | 0.186 |
R-free | 0.24434 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2q2f |
RMSD bond length | 0.019 |
RMSD bond angle | 1.943 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.580 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.286 | |
Number of reflections | 3854 | |
<I/σ(I)> | 11 | |
Completeness [%] | 92.0 | |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 288 | 0.1 M MES, pH 6.0, 20 % 2-propanol, 20 % PEG MME 2000 |