5KIM
PSEUDO T4 LYSOZYME MUTANT - Y88PHE-I
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-003 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-04-20 |
Detector | DECTRIS PILATUS 200K |
Wavelength(s) | 1.54 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 60.283, 60.283, 96.197 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.374 - 1.500 |
R-factor | 0.1619 |
Rwork | 0.160 |
R-free | 0.18440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1l63 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.817 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.530 |
High resolution limit [Å] | 1.500 | 4.070 | 1.500 |
Rmerge | 0.058 | 0.043 | 0.341 |
Rmeas | 0.060 | ||
Rpim | 0.014 | ||
Total number of observations | 431722 | ||
Number of reflections | 33148 | ||
<I/σ(I)> | 23.4 | ||
Completeness [%] | 99.9 | 99.4 | 99.1 |
Redundancy | 13 | 21.6 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 291.15 | 2.0M KPO4, 50mM 2-HYDROXYETHYLDISULFID, 50mM 2-MERCAPTOETHANOL, PH6.9 |