5KII
PSEUDO T4 LYSOZYME MUTANT - Y88PHE-METHYL
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-003 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-03-01 |
Detector | DECTRIS PILATUS 200K |
Wavelength(s) | 1.54 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 60.183, 60.183, 95.834 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.787 - 1.560 |
R-factor | 0.2014 |
Rwork | 0.200 |
R-free | 0.22290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1l63 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.774 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.590 |
High resolution limit [Å] | 1.560 | 4.230 | 1.560 |
Rmerge | 0.065 | 0.037 | 0.783 |
Rmeas | 0.068 | ||
Rpim | 0.017 | ||
Total number of observations | 361463 | ||
Number of reflections | 29084 | ||
<I/σ(I)> | 18 | ||
Completeness [%] | 99.1 | 100 | 98 |
Redundancy | 12.4 | 26.9 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 291.15 | 2.0M KP04, 50mM 2-HYDROXYETHYLDISULFIDE, 50mM 2-MERCAPTOETHANOL, PH 6.9 |