5KIG

PSEUDO T4 LYSOZYME MUTANT - Y88F

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-003
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-15
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54
Spacegroup name	P 32 2 1
Unit cell lengths	60.368, 60.368, 96.571
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	22.988 - 1.500
R-factor	0.1645
Rwork	0.163
R-free	0.19560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1l63
RMSD bond length	0.005
RMSD bond angle	0.797
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.530
High resolution limit [Å]	1.500	4.070	1.500
Rmerge	0.061	0.032	0.399
Rmeas	0.064
Rpim	0.021
Total number of observations	269948
Number of reflections	33527
<I/σ(I)>	18.2
Completeness [%]	99.3	99.1	94.8
Redundancy	8.1	14.2	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.9	291.15	2.0M KPO4, 50MM 2-HYDROXYETHYLDISULFIDE, 50MM 2-MERCAPTOETHANOL, PH6.9