5KI2
PSEUDO T4 LYSOZYME MUTANT - Y18PHE-METHYL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-003 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-01-06 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 60.197, 60.197, 95.969 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 18.760 - 1.500 |
| R-factor | 0.19 |
| Rwork | 0.188 |
| R-free | 0.22100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l63 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.014 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (DEV_1839) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.760 | 1.550 |
| High resolution limit [Å] | 1.499 | 1.499 |
| Rmerge | 0.115 | 0.757 |
| Number of reflections | 31673 | |
| <I/σ(I)> | 23.1 | 1.8 |
| Completeness [%] | 96.0 | 67 |
| Redundancy | 9.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 291.15 | 2.2M KPO4, 50MM 2-HYDROXYETHYLDISULFIDE, 50MM 2-MERCAPTOETHANOL, PH 6.8 |
