5KHZ
PSEUDO T4 LYSOZYME
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-003 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-06-13 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 60.248, 60.248, 96.454 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 21.980 - 1.490 |
| R-factor | 0.173 |
| Rwork | 0.171 |
| R-free | 0.20100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l63 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.998 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (DEV_1951) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 21.980 |
| High resolution limit [Å] | 1.490 |
| Rmerge | 0.085 |
| Number of reflections | 31370 |
| <I/σ(I)> | 28.4 |
| Completeness [%] | 92.0 |
| Redundancy | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 291.15 | 2.0M KPO4, 50MM 2-HYDROXYETHYLDISULFIDE, 50MM 2-MERCAPTOETHANOL, PH 6.9 |
