5KHS

Crystal structures of the Burkholderia multivorans hopanoid transporter HpnN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-27
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.98
Spacegroup name	P 1 21 1
Unit cell lengths	112.158, 143.374, 112.661
Unit cell angles	90.00, 114.10, 90.00

Refinement procedure

Resolution	83.567 - 3.758
R-factor	0.2652
Rwork	0.263
R-free	0.30730
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.641
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	100.000	100.000	3.920
High resolution limit [Å]	3.758	7.840	3.758
Rmerge	0.075	0.041
Rmeas	0.089
Rpim	0.050
Total number of observations	122869
Number of reflections	32876
<I/σ(I)>	6.8
Completeness [%]	99.3	98.9	99.3
Redundancy	3.7	3.6	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	3.5	298	18% PEG2000, 0.2M LiSo4,