5KHQ
Rasip1 RA domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-05-21 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979460 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 89.200, 89.200, 177.950 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.050 - 2.800 |
| R-factor | 0.2358 |
| Rwork | 0.234 |
| R-free | 0.26180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5kho |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.485 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.000 |
| High resolution limit [Å] | 2.800 | 8.300 | 2.800 |
| Rmerge | 0.106 | 0.028 | 2.814 |
| Number of reflections | 10877 | ||
| <I/σ(I)> | 26.35 | 82.09 | 2.1 |
| Completeness [%] | 99.5 | 98.8 | 97.6 |
| Redundancy | 24.8 | 25.7 | |
| CC(1/2) | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 1.5M Ammonium Sulfate and MES pH 6.0 |
