5KHN

Crystal structures of the Burkholderia multivorans hopanoid transporter HpnN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-27
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.98
Spacegroup name	P 1 21 1
Unit cell lengths	111.914, 129.546, 111.728
Unit cell angles	90.00, 114.04, 90.00

Refinement procedure

Resolution	93.803 - 3.445
R-factor	0.2848
Rwork	0.283
R-free	0.31900
Structure solution method	SIRAS
RMSD bond length	0.003
RMSD bond angle	0.604
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	100.000	100.000	3.570
High resolution limit [Å]	3.450	7.430	3.450
Rmerge	0.126	0.080
Rmeas	0.132	0.084
Rpim	0.051	0.026	0.712
Total number of observations	424764
Number of reflections	38588
<I/σ(I)>	7.3
Completeness [%]	99.9	99.9	99.9
Redundancy	11	11.2	10.7
CC(1/2)		0.995	0.673

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	3.5	298	18% PEG2000, and 0.2M LiSo4