5KHD

Structure of 1.75 A BldD C-domain-c-di-GMP complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.11
Spacegroup name	C 2 2 21
Unit cell lengths	36.160, 95.580, 99.580
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.977 - 1.750
R-factor	0.1832
Rwork	0.183
R-free	0.18500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.036
RMSD bond angle	3.138
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall
Low resolution limit [Å]	27.977
High resolution limit [Å]	1.750
Number of reflections	17238
<I/σ(I)>	10
Completeness [%]	96.5
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	Potassium/sodium phosphate, pH 6.5