5KGO
Structure of K. pneumonia MrkH-c-di-GMP complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-01-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.11 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.573, 74.569, 135.538 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.285 - 2.900 |
| R-factor | 0.2318 |
| Rwork | 0.226 |
| R-free | 0.28870 |
| Structure solution method | MAD |
| RMSD bond length | 0.028 |
| RMSD bond angle | 1.721 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.285 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.800 | |
| Number of reflections | 14247 | |
| <I/σ(I)> | 5.2 | 1.2 |
| Completeness [%] | 94.1 | 96.5 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 20% PEG 3350, 0.1 M Bis tris propane/HCl pH 6.5, 0.2 M sodium fluoride. |
