5KGM

2.95A resolution structure of Apo independent phosphoglycerate mutase from C. elegans (monoclinic form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-12-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00000
Spacegroup name	P 1 21 1
Unit cell lengths	67.850, 94.375, 102.201
Unit cell angles	90.00, 96.57, 90.00

Refinement procedure

Resolution	42.982 - 2.950
R-factor	0.1868
Rwork	0.184
R-free	0.23740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1o98
RMSD bond length	0.008
RMSD bond angle	1.013
Data reduction software	XDS
Data scaling software	Aimless (0.2.14)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.480	46.480	3.130
High resolution limit [Å]	2.950	8.850	2.950
Rmerge	0.141	0.036	0.625
Rmeas	0.168
Rpim	0.090
Total number of observations	90718
Number of reflections	27020
<I/σ(I)>	9
Completeness [%]	99.7	98.3	99.9
Redundancy	3.4	3.5	3.5
CC(1/2)	0.985

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	30% (w/v) PEG 5000 MME, 0.1 M MES , 0.2 M ammonium sulfate