5KFS
Human DNA polymerase eta R61A-DNA ternary complex: ground state at pH7.0 (K+ MES) with 1 Ca2+ ion
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-07-03 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 61 |
Unit cell lengths | 98.710, 98.710, 82.080 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.950 - 1.460 |
R-factor | 0.178 |
Rwork | 0.176 |
R-free | 0.20500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ecq |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.950 | 1.500 |
High resolution limit [Å] | 1.460 | 1.460 |
Rmerge | 0.065 | 0.941 |
Number of reflections | 78686 | |
<I/σ(I)> | 19.76 | 2.18 |
Completeness [%] | 100.0 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 6 | 298 | 17% PEG 2000MME, 0.1 M MES |