5KFG

Human DNA polymerase eta-DNA ternary complex: reaction with 10 mM Mn2+ for 30s

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-03
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 61
Unit cell lengths	98.380, 98.380, 82.160
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.968 - 1.550
R-factor	0.176
Rwork	0.174
R-free	0.20420
Starting model (for MR)	4ecq
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.968		1.590
High resolution limit [Å]	1.550	6.930	1.550
Rmerge	0.056	0.020	0.657
Rmeas	0.060	0.022	0.712
Total number of observations	450906
Number of reflections	65402	731	4830
<I/σ(I)>	24.18	80.79	3.32
Completeness [%]	99.9	94.7	100
CC(1/2)	1.000	0.999	0.841

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6	298	17% PEG 2000MME, 0.1 M MES