5KFE

Human DNA polymerase eta-DNA ternary complex: reaction with 1 mM Mn2+ for 600s

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-03
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 61
Unit cell lengths	98.620, 98.620, 81.930
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.918 - 1.550
R-factor	0.1752
Rwork	0.173
R-free	0.20310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ecq
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]			1.590
High resolution limit [Å]	1.550	6.930	1.550
Rmerge	0.059	0.019	0.651
Rmeas	0.063	0.021	0.700
Total number of observations	491628
Number of reflections	65580	741	4857
<I/σ(I)>	23.65	83.77	3.15
Completeness [%]	99.9	95.6	100
CC(1/2)	1.000	0.999	0.829

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6	298	17% PEG 2000MME, 0.1 M MES