5KF4
Crystal structure of FN3 domain (Residues P368-P466) of Human collagen XX
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-12-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.476, 78.597, 81.989 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.864 - 2.500 |
R-factor | 0.2587 |
Rwork | 0.258 |
R-free | 0.28370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3tes |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.500 | 2.620 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.049 | 0.186 |
Number of reflections | 14950 | |
<I/σ(I)> | 22 | 12 |
Completeness [%] | 98.1 | 99.9 |
Redundancy | 6.8 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 80mM Magnesium Acetate, 50mM Sodium Cacodylate (pH6.5), 30%(w/v) PEG4000 |