5KF4

Crystal structure of FN3 domain (Residues P368-P466) of Human collagen XX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	56.476, 78.597, 81.989
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.864 - 2.500
R-factor	0.2587
Rwork	0.258
R-free	0.28370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3tes
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.500	2.620
High resolution limit [Å]	2.500	2.500
Rmerge	0.049	0.186
Number of reflections	14950
<I/σ(I)>	22	12
Completeness [%]	98.1	99.9
Redundancy	6.8	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	80mM Magnesium Acetate, 50mM Sodium Cacodylate (pH6.5), 30%(w/v) PEG4000