5KF3
Truncated hemolysin A from P. mirabilis Y134A at 2.2 Angstroms resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-06-20 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 33.906, 59.775, 115.766 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.539 - 2.200 |
R-factor | 0.1744 |
Rwork | 0.173 |
R-free | 0.20690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4w8q |
RMSD bond length | 0.004 |
RMSD bond angle | 0.641 |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.5.6) |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.240 |
High resolution limit [Å] | 2.200 | 5.970 | 2.200 |
Rmerge | 0.094 | 0.057 | 0.207 |
Number of reflections | 12612 | ||
<I/σ(I)> | 8.9 | ||
Completeness [%] | 99.7 | 99.5 | 98.4 |
Redundancy | 8.8 | 8.9 | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 100 MM CITRATE PH 5.5, 100 MM NACL, PEG 4000 (8 - 16%) |