5KF1
X-ray structure of a glucosamine N-Acetyltransferase from Clostridium acetobutylicum, apo form, pH 5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-10 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.864, 65.694, 90.486 |
| Unit cell angles | 90.00, 106.93, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.187 |
| Rwork | 0.184 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | MODEL FROM SAD DATA SET |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.763 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.070 | 0.309 |
| Number of reflections | 46396 | |
| <I/σ(I)> | 10 | 1.9 |
| Completeness [%] | 95.4 | 87.2 |
| Redundancy | 3.5 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 8-11% PEG-8000, 100 mH HOMOPIPES |
