5KDG
Crystal Structure of Salmonella Typhimurium Effector GtgE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-22 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.6362 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 64.302, 64.302, 124.206 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.100 - 1.730 |
| R-factor | 0.21051 |
| Rwork | 0.209 |
| R-free | 0.23813 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mi7 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.009 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.100 | 1.760 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmerge | 0.684 | |
| Number of reflections | 26504 | |
| <I/σ(I)> | 37.8 | 2.7 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 6.7 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 1.8 M ammonium sulfate, 0.1 M Bis-Tris (pH 5.5) and 20% (w/v) glycerol |
