5KDD
Apo-structure of humanised RadA-mutant humRadA22
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-10-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979500 |
| Spacegroup name | P 1 |
| Unit cell lengths | 37.849, 42.583, 86.441 |
| Unit cell angles | 78.66, 85.49, 63.63 |
Refinement procedure
| Resolution | 84.748 - 1.990 |
| R-factor | 0.2305 |
| Rwork | 0.229 |
| R-free | 0.25010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.200 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.748 | 1.994 |
| High resolution limit [Å] | 1.988 | 1.988 |
| Rmerge | 0.130 | 0.286 |
| Number of reflections | 31086 | |
| <I/σ(I)> | 5 | 2.1 |
| Completeness [%] | 95.2 | 94.5 |
| Redundancy | 1.8 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 297 | 20% PEG3350, 0.2M MgSO4 |
