5KD2
BT_4244 metallopeptidase from Bacteroides thetaiotaomicron
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97945 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 81.957, 81.957, 182.138 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 70.980 - 2.150 |
| R-factor | 0.17077 |
| Rwork | 0.169 |
| R-free | 0.20984 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.528 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.980 | 2.220 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.070 | 0.366 |
| Number of reflections | 39361 | |
| <I/σ(I)> | 15.9 | 4.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.5 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | PEG 3350, K/Na Tartrate, Tris-HCl pH 8.0 |
