5KCI
Crystal Structure of HTC1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-18 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97851 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 62.460, 62.460, 117.580 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.810 - 1.833 |
| R-factor | 0.2059 |
| Rwork | 0.201 |
| R-free | 0.25280 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.888 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.810 | 1.890 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.683 | |
| Number of reflections | 21161 | |
| <I/σ(I)> | 12.7 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.8 | 7.7 |
| CC(1/2) | 0.873 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 5.6-8.1% glycerol, 1.6-2.1 M ammonium sulfate, 0.1-0.2 M Tris. |
