5KC9

Crystal structure of the amino-terminal domain (ATD) of iGluR Delta-1 (GluD1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-04-28
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.91730
Spacegroup name	P 32 2 1
Unit cell lengths	128.420, 128.420, 153.230
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	59.220 - 2.300
R-factor	0.2006
Rwork	0.199
R-free	0.22730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5kc8
RMSD bond length	0.004
RMSD bond angle	0.855
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (dev_1772)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.220	2.360
High resolution limit [Å]	2.300	2.300
Rmerge	0.051	1.222
Number of reflections	65080
<I/σ(I)>	22.6	1.9
Completeness [%]	99.7	98.5
Redundancy	8.5	8.7
CC(1/2)	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M MES (2-ethanesulfonic acid)/imidazole pH 6.5, 10% (w/v) polyethylene glycol 8000, 20% (w/v) ethylene glycol, 0.02 M 1,6-hexanediol, 0.02 M (RS)-1,2-propanediol, 0.02 M 1-butanol, 0.02 M 2-propanol, 0.02 M 1,4-butanediol and 0.02 M 1,3-propanediol