5KC8

Crystal structure of the amino-terminal domain (ATD) of iGluR Delta-2 (GluD2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-27
Detector	ADSC QUANTUM 315
Wavelength(s)	1.00000
Spacegroup name	I 2 2 2
Unit cell lengths	45.100, 79.160, 246.690
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.871 - 1.751
R-factor	0.1907
Rwork	0.189
R-free	0.21840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wjw
RMSD bond length	0.008
RMSD bond angle	0.917
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (dev_1772)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.030	1.800
High resolution limit [Å]	1.750	1.750
Rmerge	0.078	1.314
Number of reflections	44958
<I/σ(I)>	24.2	2.2
Completeness [%]	99.6	98.3
Redundancy	15.3	9.5
CC(1/2)	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% (w/v) polyethylene glycol 1000, 0.2 M calcium Acetate, 0.1 M imidazole pH 8.0 and 0.4 M NDSB-221 (non-detergent sulfobetaine 221)