5KC7
Crystal structure of Cbln1 (Val55-Gly58 deletion mutant)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-04-24 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97625 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 187.450, 187.450, 187.450 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.098 - 7.035 |
| R-factor | 0.2772 |
| Rwork | 0.270 |
| R-free | 0.35100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5kc5 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.916 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2283: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.550 | 7.220 |
| High resolution limit [Å] | 7.000 | 7.000 |
| Rmerge | 0.048 | 0.394 |
| Number of reflections | 1801 | |
| <I/σ(I)> | 28.2 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 9.4 | 9.9 |
| CC(1/2) | 0.998 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 2.75 M 1,6-Hexanediol, 50 mM Tris pH 8.0 and 5 mM magnesium sulphate |
