5KC2
Negative stain structure of Vps15/Vps34 complex
EM specimen
| Staining | Yes |
| Staining material | Uranyl acetate |
EM Data Collection
| Microscope | FEI POLARA 300 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 120 |
| Detector | GATAN ULTRASCAN 4000 (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 28 |
| Number of Particle | 14172 |
| Software | EMAN,SPIDER,IMAGIC |
| CTF correction | PHASE FLIPPING ONLY |
