5KC0
Crystal structure of TmRibU, hexagonal crystal form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-26 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.979 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 72.611, 72.611, 232.912 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.400 - 3.200 |
R-factor | 0.2681 |
Rwork | 0.266 |
R-free | 0.29830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3p5n |
RMSD bond length | 0.003 |
RMSD bond angle | 0.639 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.431 | 3.280 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.058 | 0.062 |
Number of reflections | 6009 | |
<I/σ(I)> | 31.3 | |
Completeness [%] | 91.2 | |
Redundancy | 18.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 277 | 100 mM sodium acetate, pH 4.5, 28% PEG400, and 50 mM magnesium acetate |