5KBW
Crystal structure of TmRibU, the riboflavin-binding S subunit from the Thermotoga maritima ECF transporter
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-10-22 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 40.142, 94.365, 50.986 |
Unit cell angles | 90.00, 95.81, 90.00 |
Refinement procedure
Resolution | 30.483 - 2.609 |
R-factor | 0.2055 |
Rwork | 0.202 |
R-free | 0.26490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5kc0 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.716 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.800 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 10106 | |
<I/σ(I)> | 19.8 | 2.9 |
Completeness [%] | 97.3 | 75.3 |
Redundancy | 2.8 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 5 | 293 | 100mM NaCitrate, pH 5.0, 30% PEG 500 DME, 100mM NaCl |