5KBW
Crystal structure of TmRibU, the riboflavin-binding S subunit from the Thermotoga maritima ECF transporter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.142, 94.365, 50.986 |
| Unit cell angles | 90.00, 95.81, 90.00 |
Refinement procedure
| Resolution | 30.483 - 2.609 |
| R-factor | 0.2055 |
| Rwork | 0.202 |
| R-free | 0.26490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5kc0 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.716 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.800 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 10106 | |
| <I/σ(I)> | 19.8 | 2.9 |
| Completeness [%] | 97.3 | 75.3 |
| Redundancy | 2.8 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 5 | 293 | 100mM NaCitrate, pH 5.0, 30% PEG 500 DME, 100mM NaCl |
