5KBM
Candida Albicans Superoxide Dismutase 5 (SOD5), D113N Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-10-22 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.550, 39.450, 102.850 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.679 - 1.416 |
| R-factor | 0.2193 |
| Rwork | 0.217 |
| R-free | 0.24660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4n3t |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.037 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.460 | 1.490 |
| High resolution limit [Å] | 1.410 | 1.410 |
| Rmerge | 0.411 | |
| Number of reflections | 26998 | |
| <I/σ(I)> | 16.3 | 3.2 |
| Completeness [%] | 97.0 | 88.1 |
| Redundancy | 4.6 | 4.4 |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | 0.1 M HEPES pH 7.0, 2.4 M ammonium sulfate |
