5KBJ
Structure of Rep-DNA complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-02-24 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | P 1 |
Unit cell lengths | 59.200, 77.500, 100.700 |
Unit cell angles | 80.80, 75.50, 71.90 |
Refinement procedure
Resolution | 97.100 - 3.090 |
R-factor | 0.2313 |
Rwork | 0.225 |
R-free | 0.27700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4pql |
RMSD bond length | 0.003 |
RMSD bond angle | 0.641 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 97.100 |
High resolution limit [Å] | 3.090 |
Number of reflections | 28484 |
<I/σ(I)> | 7 |
Completeness [%] | 94.5 |
Redundancy | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 28% PEG 400, 0.1 M sodium/potassium phosphate (pH 6.9), 4% PEG 3350, and 10% polyethylene glycol |