5KBD

Structural Studies of Transcription Factor p73 DNA Binding Domain Bound to PA26 20-mer Response Element

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-02-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.98
Spacegroup name	P 61
Unit cell lengths	174.878, 174.878, 34.282
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.411 - 2.800
R-factor	0.2817
Rwork	0.279
R-free	0.30760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4g82
RMSD bond length	0.003
RMSD bond angle	0.546
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.10.1_2155)
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.411	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.084	0.737
Number of reflections	15238
<I/σ(I)>	20.26	1.48
Completeness [%]	99.3	99.5
Redundancy	4.5	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1 M Tris pH 9.0, 50 mM Sodium Acetate, 16% (w/v) PEG 3350; 2:1 or 4:1 Protein:DNA ratio, 1:1 protein/DNA solution:well solution ratio