5KBD
Structural Studies of Transcription Factor p73 DNA Binding Domain Bound to PA26 20-mer Response Element
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-02-04 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.98 |
Spacegroup name | P 61 |
Unit cell lengths | 174.878, 174.878, 34.282 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.411 - 2.800 |
R-factor | 0.2817 |
Rwork | 0.279 |
R-free | 0.30760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4g82 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.546 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.10.1_2155) |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.411 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.084 | 0.737 |
Number of reflections | 15238 | |
<I/σ(I)> | 20.26 | 1.48 |
Completeness [%] | 99.3 | 99.5 |
Redundancy | 4.5 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M Tris pH 9.0, 50 mM Sodium Acetate, 16% (w/v) PEG 3350; 2:1 or 4:1 Protein:DNA ratio, 1:1 protein/DNA solution:well solution ratio |