5KBA
Computational Design of Self-Assembling Cyclic Protein Homooligomers
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-09-24 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 93.372, 48.817, 139.267 |
Unit cell angles | 90.00, 98.87, 90.00 |
Refinement procedure
Resolution | 19.907 - 2.601 |
R-factor | 0.203 |
Rwork | 0.201 |
R-free | 0.23880 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.738 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 2.600 |
Number of reflections | 19299 |
<I/σ(I)> | 14.5 |
Completeness [%] | 98.0 |
Redundancy | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.4 M Sodium Chloride, 0.1 M Tris-HCl pH 8.5 and 30 % PEG 3,350 |