5KAZ
Human SH2D1B structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-09 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 66.983, 66.983, 105.155 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.191 - 1.700 |
| R-factor | 0.1907 |
| Rwork | 0.189 |
| R-free | 0.21430 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.209 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 31.054 |
| High resolution limit [Å] | 1.700 |
| Number of reflections | 13571 |
| <I/σ(I)> | 33.9 |
| Completeness [%] | 98.8 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.1 M Na-Citrate pH 5.5, 24% PEG 4000, 50 mM (NH)2SO4 |
