5KAK

Crystal structure of an uncharacterized NIPSNAP-like domain protein from Burkholderia xenovorans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-13
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 1 21 1
Unit cell lengths	70.630, 70.830, 109.020
Unit cell angles	90.00, 98.62, 90.00

Refinement procedure

Resolution	43.426 - 1.650
R-factor	0.2066
Rwork	0.206
R-free	0.25050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vqy
RMSD bond length	0.006
RMSD bond angle	0.911
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX ((dev_2386: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.690
High resolution limit [Å]	1.650	1.650
Rmerge	0.090	0.457
Number of reflections	127463
<I/σ(I)>	10.32	2.66
Completeness [%]	99.7	100
Redundancy	4.08	4.07
CC(1/2)	0.994

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	290	tray270444 JCSG C12: 10%Peg1000, 10%Peg8000; cryo: 20% ethylene glycol; BuxeA.00127.c.B1.PS37846 at 24.2 mg/ml, puck sbt3-7