5K9X

Crystal structure of Tryptophan synthase alpha chain from Legionella pneumophila subsp. pneumophila

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97924
Spacegroup name	P 21 21 21
Unit cell lengths	47.021, 71.046, 71.818
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.211 - 2.016
R-factor	0.1933
Rwork	0.191
R-free	0.23790
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.658
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	HKL-3000
Refinement software	PHENIX (dev_2056)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.030
High resolution limit [Å]	2.000	5.430	2.000
Rmerge	0.058	0.029	0.893
Number of reflections	16344
<I/σ(I)>	10.8
Completeness [%]	99.4	98.5	93.1
Redundancy	11.3	10.6	8.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.01 M Sodium citrate,33% PEG 6000,