5K9O
Crystal structure of multidonor HV1-18+HD3-9 class broadly neutralizing Influenza A antibody 31.b.09 in complex with Hemagglutinin H1 A/California/04/2009
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-05-23 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | P 6 2 2 |
Unit cell lengths | 208.648, 208.648, 252.031 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.965 - 3.387 |
R-factor | 0.294 |
Rwork | 0.291 |
R-free | 0.34560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wt5 3al4 3lzg 3ube 3ubj 3ubn 3ubq 3ztn 4jtv 4jtx 4juo 4lss |
RMSD bond length | 0.004 |
RMSD bond angle | 0.841 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.380 |
Number of reflections | 29074 |
<I/σ(I)> | 11.76 |
Completeness [%] | 71.0 |
Redundancy | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1M imidazole, pH 6.5, 0.1M MgCl2, 1.75M NaCl, and 15% PEG-3350 |