5K9N
Structural and Mechanistic Analysis of Drosophila melanogaster Polyamine N acetyltransferase, an enzyme that Catalyzes the Formation of N acetylagmatine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2015-08-17 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.110, 72.350, 59.520 |
| Unit cell angles | 90.00, 109.64, 90.00 |
Refinement procedure
| Resolution | 44.313 - 2.300 |
| R-factor | 0.2143 |
| Rwork | 0.208 |
| R-free | 0.28780 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.181 |
| Data reduction software | iMOSFLM |
| Refinement software | PHENIX (1.6.1_357) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 56.090 |
| High resolution limit [Å] | 2.300 |
| Number of reflections | 18703 |
| <I/σ(I)> | 6.3 |
| Completeness [%] | 100.0 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 100 mM Tris , 200 mM sodium acetate, 30% PEG 4000 |
