5K9M
Crystal Structure of PriB Binary Complex with Product Diphosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-07-09 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.97931 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 41.727, 83.020, 95.304 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.224 - 1.500 |
R-factor | 0.1945 |
Rwork | 0.194 |
R-free | 0.22700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5inj |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.224 | 1.554 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.140 | 1.900 |
Number of reflections | 101546 | |
<I/σ(I)> | 8.59 | 1.7 |
Completeness [%] | 99.7 | 99.79 |
Redundancy | 1.9 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293.15 | PriB was crystallized by sitting drop vapor diffusion method by 1:1 v/v mixing of 10 mg/mL PriB solution in the presence of 1 mM daptomycin and 1 mM Dimethylallyl diphosphate (DMAPP) with reservoir solution containing 0.1 M Tris pH 8, 28% w/v PEG 4000. The mixed drops of 0.4 uL were equilibrated over a reservoir solution of 50 uL and incubated at 20oC in the dark. |