5K9L

Beclin 2 CCD N187L mutant homodimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-17
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	C 1 2 1
Unit cell lengths	127.971, 44.709, 94.899
Unit cell angles	90.00, 130.57, 90.00

Refinement procedure

Resolution	72.089 - 2.520
Rwork	0.235
R-free	0.27820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.018
RMSD bond angle	1.381
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.090	2.620
High resolution limit [Å]	2.520	2.520
Number of reflections	13749
<I/σ(I)>	6.1	1.1
Completeness [%]	97.1	86.9
Redundancy	3.7	3.4
CC(1/2)	0.991

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.1 M MgCl2 and 19% PEG3350