5K9J

Crystal structure of multidonor HV6-1-class broadly neutralizing Influenza A antibody 56.a.09 isolated following H5 immunization.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-30
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1
Spacegroup name	P 21 21 2
Unit cell lengths	78.790, 103.758, 59.983
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.124 - 1.904
R-factor	0.197
Rwork	0.195
R-free	0.23120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bvk 4lss
RMSD bond length	0.004
RMSD bond angle	0.770
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.900
Number of reflections	25791
<I/σ(I)>	18.29
Completeness [%]	99.0
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	0.1M sodium acetate, pH 5.5, 25% (w/v) PEG-400 and 11% (w/v) PEG-8000