5K99
Crystal structure of microcin immunity protein MccF from Bacillus anthracis in complex with McC
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-11-18 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9794 |
Spacegroup name | P 43 |
Unit cell lengths | 118.275, 118.275, 55.698 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.266 - 1.500 |
R-factor | 0.1331 |
Rwork | 0.132 |
R-free | 0.15330 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4jvo |
RMSD bond length | 0.011 |
RMSD bond angle | 1.216 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 28.266 |
High resolution limit [Å] | 1.500 |
Rmerge | 0.032 |
Number of reflections | 189029 |
<I/σ(I)> | 19 |
Completeness [%] | 96.0 |
Redundancy | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1 M Succinic acid ph 7.0, 15% Peg 3350 |