5K8U
Crystal structure of ZIKV NS3 helicase in complex with ADP and Mn2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2016-04-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.490, 53.220, 64.950 |
| Unit cell angles | 90.00, 105.31, 90.00 |
Refinement procedure
| Resolution | 40.170 - 1.601 |
| R-factor | 0.1712 |
| Rwork | 0.170 |
| R-free | 0.21050 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.884 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.600 |
| Number of reflections | 50909 |
| <I/σ(I)> | 18.6 |
| Completeness [%] | 96.6 |
| Redundancy | 5.29 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 10% PEG 6000, 5% MPD 0.1M, HEPES pH 7.5 |
