5K8M
Apo 5-nitroanthranilate aminohydrolase
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-11-07 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9793 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 175.270, 175.270, 354.090 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 78.540 - 2.750 |
R-factor | 0.1792 |
Rwork | 0.178 |
R-free | 0.21350 |
Structure solution method | MAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.482 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 78.540 |
High resolution limit [Å] | 2.540 |
Number of reflections | 69712 |
<I/σ(I)> | 10.41 |
Completeness [%] | 94.8 |
Redundancy | 10.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 1.07 M Monosodium phosphate, 0.83 M Dipotassium phosphate |