5K8L
Crystal structure of ZIKV NS3 helicase in complex with GTP-gammar S
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 110 |
Detector technology | PIXEL |
Collection date | 2016-04-28 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 64.860, 51.750, 66.200 |
Unit cell angles | 90.00, 105.77, 90.00 |
Refinement procedure
Resolution | 31.628 - 1.751 |
R-factor | 0.1887 |
Rwork | 0.186 |
R-free | 0.24010 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.865 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.750 |
Number of reflections | 42472 |
<I/σ(I)> | 11.23 |
Completeness [%] | 99.2 |
Redundancy | 5.93 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 10% PEG 6000, 5% MPD, 0.1M HEPES pH 7.5 |