5K8J

Structure of Caulobacter crescentus VapBC1 (apo form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-3
Synchrotron site	MAX II
Beamline	I911-3
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-30
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.972
Spacegroup name	P 31 2 1
Unit cell lengths	86.290, 86.290, 113.820
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.400 - 1.600
R-factor	0.1582
Rwork	0.158
R-free	0.16810
Structure solution method	SAD
RMSD bond length	0.014
RMSD bond angle	1.134
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2152: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.400	1.640
High resolution limit [Å]	1.600	1.600
Number of reflections	125126
<I/σ(I)>	22.8	1.7
Completeness [%]	100.0	100
Redundancy	23.2	22.8
CC(1/2)	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	292.15	3 M NaCl, 0.1 M Bis-tris pH 5.5, 5 mM MgCl2, 5 mM beta-mercapto ethanol, 25% glycerol