5K8I
Crystal structure of ZIKV NS3 helicase in complex with ATP and Mn2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2016-04-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.510, 53.170, 65.110 |
| Unit cell angles | 90.00, 105.31, 90.00 |
Refinement procedure
| Resolution | 39.005 - 1.694 |
| R-factor | 0.1809 |
| Rwork | 0.179 |
| R-free | 0.21630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.917 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.690 |
| Number of reflections | 44048 |
| <I/σ(I)> | 13.03 |
| Completeness [%] | 95.6 |
| Redundancy | 2.98 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 10% PEG 6000, 5% MPD, 0.1M HEPES pH 7.5 |
