5K8E
Xylooligosaccharide oxidase from Myceliophthora thermophila C1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR-H |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-02-09 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 123.951, 59.440, 68.693 |
| Unit cell angles | 90.00, 90.62, 90.00 |
Refinement procedure
| Resolution | 53.600 - 1.930 |
| R-factor | 0.16056 |
| Rwork | 0.159 |
| R-free | 0.19544 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ZR6 and 3RJ8 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.327 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.600 | 1.970 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.163 | 0.673 |
| Number of reflections | 37594 | |
| <I/σ(I)> | 11.8 | 2.4 |
| Completeness [%] | 99.4 | 91.4 |
| Redundancy | 7.2 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 294 | 15-18% PEG3350, 0.1 M MES buffer, 0.2 M ammonium chloride |
