5K83
Crystal Structure of a Primate APOBEC3G N-Domain, in Complex with ssDNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 105.720, 83.311, 105.754 |
| Unit cell angles | 90.00, 120.00, 90.00 |
Refinement procedure
| Resolution | 45.792 - 2.390 |
| R-factor | 0.1885 |
| Rwork | 0.186 |
| R-free | 0.25090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5k81 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.169 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.792 | 2.480 |
| High resolution limit [Å] | 2.390 | 2.390 |
| Rmerge | 0.076 | 0.430 |
| Number of reflections | 63261 | |
| <I/σ(I)> | 26 | 4.2 |
| Completeness [%] | 100.0 | 99.7 |
| Redundancy | 6.9 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M Tris pH 8.0, 6% PEG 8000 |
