5K82
Crystal Structure of a Primate APOBEC3G N-Terminal Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.99997 |
| Spacegroup name | P 32 |
| Unit cell lengths | 152.395, 152.395, 79.778 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.295 - 2.913 |
| R-factor | 0.2251 |
| Rwork | 0.224 |
| R-free | 0.25060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5k81 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.658 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.295 | 2.950 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.116 | 0.677 |
| Number of reflections | 44596 | |
| <I/σ(I)> | 29.4 | 1.9 |
| Completeness [%] | 98.8 | 85.7 |
| Redundancy | 7 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 1.9 M AmSO4, 0.1M Tris pH 8.0, 0.5 M NDSB-195 |
