5K81
Crystal Structure of a Primate APOBEC3G N-Terminal Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.999 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 106.213, 81.297, 106.298 |
| Unit cell angles | 90.00, 120.03, 90.00 |
Refinement procedure
| Resolution | 46.014 - 2.006 |
| R-factor | 0.1884 |
| Rwork | 0.188 |
| R-free | 0.22340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4j4j |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.137 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.014 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.074 | 0.583 |
| Number of reflections | 102317 | |
| <I/σ(I)> | 18.6 | 2.5 |
| Completeness [%] | 97.8 | 82.1 |
| Redundancy | 4.4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2 M Na K tartrate, 15% PEG 4000 |
